Identification of a novel hydrolase encoded by hy-1 from Bacillus amyloliquefaciens for bioremediation of carbendazim contaminated soil and food

Ying Li, Miaomiao Chi, Xizhen Ge

Abstract


Carbendazim (MBC) is an effective antifungal and antibacterial pesticide in agricultural applications. However, the MBC-contaminated soil and food are difficult to be restored. In this work, a novel MBC-hydrolase HY-1 encoded by gene hy-1 from an isolated MBC-degrading bacteria Bacillus amyloliquefaciens has been screened and identified. The 858 bp hydrolase gene was expressed in E. coli BL21 and the 32 kDa hydrolase HY-1 was purified. The purified HY-1 was able to catalyze MBC into 2-aminobenzimidazole (2-AB) without the need for any cofactors. Then bioremediation experiment was conducted and both the strain Car4 and cell crude extract of E. coli (pET-hy1) accelerated MBC degradation in soil. Moreover, purified HY-1 was available in removing MBC residue on the surface of cucumber. This work explored the possibility of microbial and enzymatic bioremediation on MBC-contaminated soil and food, provide a new way for bioremediation of pesticide contaminations.
Keywords: carbendazim, bioremediation, antifungal and antibacterial pesticide, hydrolase, soil, food, pesticide contamination
DOI: 10.25165/j.ijabe.20191202.4190

Citation: Y Li, M M Chi, X Z Ge. Identification of a novel hydrolase encoded by hy-1 from Bacillus amyloliquefaciens for bioremediation of carbendazim contaminated soil and food. Int J Agric & Biol Eng, 2019; 12(2): 218–224.

Keywords


carbendazim, bioremediation, antifungal and antibacterial pesticide, hydrolase, soil, food, pesticide contamination

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References


Boudina A, Emmelin C, Baaliouamer A, Grenier-Loustalot M F, Chovelon J M. Photochemical behaviour of carbendazim in aqueous solution. Chemosphere, 2003; 50(5): 649–655.

Wang X, Song M, Wang Y, Gao C. Response of soil bacterial community to repeated applications of carbendazim. Ecotoxicology & Environmental Safety, 2012; 75(1): 33–39.

Mazellier P, Leroy É, Laat J D, Legube B. Degradation of carbendazim by UV/H2O2 investigated by kinetic modelling. Environmental Chemistry Letters, 2003; 1(1): 68–72.

Moser T, van Gestel C A, Jones S E, Koolhaas J E, Rodrigues J M, Römbke J. Ring-testing and field-validation of a terrestrial model ecosystem (TME)-an instrument for testing potentially harmful substances: effects of carbendazim on enchytraeids. Ecotoxicology, 2004; 13(1-2): 29–42.

Arora S, Mukherjee I, Trivedi T P. Determination of pesticide residue in soil, water and grain from IPM and Non-IPM field trials of rice. Bulletin of Environmental Contamination & Toxicology, 2008; 81(4): 373–376.

Selmanoglu G, Barlas N, Songur S, Kockaya E A. Carbendazim-induced haematological, biochemical and histopathological changes to the liver and kidney of male rats. Human & Experimental Toxicology, 2001; 20(12): 625–630.

Zhang G S, Jia X M, Cheng T F, Ma X H, Zhao Y H. Isolation and characterization of a new carbendazim-degrading Ralstonia sp. strain. World Journal Of Microbiology & Biotechnology, 2005; 21(3): 265–269.

Pandey G, Dorrian S, Russell R, Brearley C, Kotsonis S, Oakeshott J. Cloning and biochemical characterization of a novel carbendazim (Methyl-1H-Benzimidazol-2-ylcarbamate)-Hydrolyzing esterase from the newly isolated Nocardioides sp strain SG-4G and its potential for use in enzymatic bioremediation. Applied & Environmental Microbiology, 2010; 76(9): 2940–2945.

Lei J, Wei S, Ren L, Hu S, Chen P. Hydrolysis mechanism of carbendazim hydrolase from the strain Microbacterium sp. djl-6F. Journal of Environmental Sciences, 2017; 54(4): 171–177.

Salunkhe V P, Sawant I S, Banerjee K, Wadkar P N, Sawant S D, Hingmire S A. Kinetics of degradation of carbendazim by B. subtilis strains: possibility of in situ detoxification. Environmental Monitoring & Assessment, 2014; 186(12): 8599–8610.

Zhang L Z, Qiao X W, Ma L P, Wang J L, Pan X L. Influence of environmental factors on degradation of carbendazim by bacillus pumilus strain NY97-1. International Journal of Environment & Pollution, 2009; 38(3): 309–317.

Das S, Mishra J, Das S K, Pandey S, Rao D S, Chakraborty A, et al. Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil. Chemosphere, 2014; 96(2): 112–121.

Das S, Pandey S, Pradhan S K, Sudarshan M, Chakraborty A, Thatoi H N. Genomic analysis and comparative hexavalent chromium reduction potential of predominant bacillus species isolated from chromite mine soil. Journal of Soil Contamination, 2015; 24(2): 206–221.

Holtman M A, Kobayashi D Y. Identification of Rhodococcuserythropolis isolates capable of degrading the fungicide carbendazim. Applied Microbiology & Biotechnology, 1997; 47(5): 578–582.

Krispin O, Allmansberger R. The Bacillus subtilis AraE protein displays a broad substrate specificity for several different sugars. Journal of Bacteriology, 1998; 180(12): 3250–3252.

Goyal N, Gupta J K, Soni S K. A novel raw starch digesting thermostable α-amylase from Bacillus sp. I-3 and its use in the direct hydrolysis of raw potato starch. Enzyme & Microbial Technology, 2005; 37(7): 723–734.

Zhang J, Yin J G, Hang B J, Cai S, He J, Zhou S G, et al. Cloning of a novel arylamidase gene from Paracoccus sp. strain FLN-7 that hydrolyzes amide pesticides. Appl Environ Microbiol, 2012; 78(14): 4848–4855.

Yarden O, Salomon R, Katan J, Aharonson N. Involvement of fungi and bacteria in enhanced and nonenhanced biodegradation of carbendazim and other benzimidazole compounds in soil. Canadian Journal of Microbiology, 1990; 36(1): 15–23.

Guo X, Li D. Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs. Antonie Van Leeuwenhoek, 2006; 90(2): 139–146.

Nautiyal C, Srivastava S, Chauhan P, Seem K, Mishra A, Sopory S. Plant growth-promoting bacteria Bacillus amyloliquefaciens NBRISN13 modulates gene expression profile of leaf and rhizosphere community in rice during salt stress. Plant Physiology & Biochemistry, 2013; 66(5): 1.

Chen X, Koumoutsi A, Scholz R, Eisenreich A, Schneider K, Heinemeyer I, et al. Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42. Nature Biotechnology, 2007; 25(9): 1007–1014.

Cui T B, Chai H Y, Jiang L X. Isolation and partial characterization of an antifungal protein produced by Bacillus licheniformis BS-3. Molecules, 2012; 17(6): 7336–7347.

Sutherland T D, Horne I, Weir K M, Coppin C W, Williams M R, Selleck M, et al. Enzymatic bioremediation: from enzyme discovery to applications. Clinical & Experimental Pharmacology & Physiology, 2004; 31(11): 817–821.

Pace N R. A molecular view of microbial diversity and the biosphere. Science, 1997; 276(5313): 734–740.




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